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If no bubbles form, it is a negative result; this suggests that the organism does not produce catalase.
Note all the results in a table such as the one below.
The rate can then be worked out by Rate=30/Average Time This gives the rate in cm3 of oxygen produced per second, this is because I am timing how long it takes to produce 30cm3 of oxygen.
Catalase is an enzyme found in food such as potato and liver.
It is used for removing Hydrogen Peroxide from the cells.
To test out how the concentration of hydrogen peroxide affects the rate of reaction first set up the apparatus below. The 0% concentration of hydrogen peroxide solution is done as a control solution to show that at 0% concentration no reaction occurs.
The different concentrations of Hydrogen Peroxide are made by adding tap water to the 20% Hydrogen Peroxide in the correct amounts.The catalase test tests for the presence of catalase, an enzyme that breaks down the harmful substance hydrogen peroxide into water and oxygen.If an organism can produce catalase, it will produce bubbles of oxygen when hydrogen peroxide is added to it.Hydrogen Peroxide is the poisonous by-product of metabolism.Catalase speeds up the decomposition of Hydrogen Peroxide into water and oxygen as shown in the equations below.Add one drop of hydrogen peroxide and look for bubbles.Bubbles are a positive result for the presence of catalase.Abstract The aim of this study was to test the rate of reactivity of the enzyme catalase on hydrogen peroxide while subject to different concentrations of an inhibitor.The hypothesis was that hydrogen peroxide will be broken down by catalase into hydrogen and oxygen, where a higher concentration of inhibitor will yield less oxygen, resultant of a lower rate of reaction.The concentration of the inhibitor will therefore be the independent variable, while the amount of oxygen will be the dependent variable.There should a smaller volume of oxygen observed as the concentration of the enzyme increases.