Chromatography Research Paper

Chromatography Research Paper-31
In paper chromatography, the stationary phase is a very uniform absorbent paper.The mobile phase is a suitable liquid solvent or mixture of solvents.For simplicity we'll assume that you know the mixture can only possibly contain five of the common amino acids.

In paper chromatography, the stationary phase is a very uniform absorbent paper.The mobile phase is a suitable liquid solvent or mixture of solvents.For simplicity we'll assume that you know the mixture can only possibly contain five of the common amino acids.

Some of the ink from the message is dissolved in the minimum possible amount of a suitable solvent, and that is also spotted onto the same line.

In the diagram, the pens are labeled 1, 2 and 3, and the message ink as M.

The position of the solvent front is marked in pencil and the chromatogram is allowed to dry and is then sprayed with a solution of ninhydrin.

Ninhydrin reacts with amino acids to give colored compounds, mainly brown or purple.

The distance travelled relative to the solvent is a constant for a particular compound as long as you keep everything else constant - the type of paper and the exact composition of the solvent, for example.

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The distance travelled relative to the solvent is called the R values because you are making a direct comparison just by looking at the chromatogram.The paper is then stood in a suitable solvent and left to develop as before.In the diagram, the mixture is M, and the known amino acids are labeled 1 to 5.You are making the assumption that if you have two spots in the final chromatogram which are the same color and have travelled the same distance up the paper, they are most likely the same compound.It isn't necessarily true of course - you could have two similarly colored compounds with very similar R In some cases, it may be possible to make the spots visible by reacting them with something which produces a colored product.You can also see that pen 1 contains a mixture of two different blue dyes - one of which might be the same as the single dye in pen 3.R values Some compounds in a mixture travel almost as far as the solvent does; some stay much closer to the base line.They all have a stationary phase (a solid, or a liquid supported on a solid) and a mobile phase (a liquid or a gas).The mobile phase flows through the stationary phase and carries the components of the mixture with it. We'll look at the reasons for this further down the page.Chromatography is used to separate mixtures of substances into their components.All forms of chromatography work on the same principle.

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