Photosynthesis Research Paper

Photosynthesis Research Paper-64
Using intact thylakoid membrane samples, we show how conventional TA kinetic and spectral analyses enable the determination of the appropriate wavelength and time delay for snapshot TA experiments.

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The findings indicated that the intracellular redox state is actively controlled to change in a 24-h cycle under constant light conditions by the circadian clock system.

Near-infrared in vitro measurements of photosystem I cofactors and electron-transfer partners with a recently developed spectrophotometer A kinetic-LED-array-spectrophotometer (Klas) was recently developed for measuring in vivo redox changes of P700, plastocyanin (PCy), and ferredoxin (Fd) in the near-infrared (NIR).

Based on these findings, we discuss the function and structural organization of green algal LHCII antennae.

The endogenous redox rhythm is controlled by a central circadian oscillator in cyanobacterium Synechococcus elongatus PCC7942 The intracellular redox and the circadian clock in photosynthetic organisms are two major regulators globally affecting various biological functions.

Although the trimeric structure of LHCII is conserved between the two lineages, its subunit composition and function are believed to differ significantly.

In this study, we purified four LHCII trimers from the green alga and analyzed their biochemical properties.This spectrophotometer is used in the present work for in vitro light-induced measurements with various combinations of photosystem I (PSI) from tobacco and two different cyanobacteria, spinach plastocyanin, cyanobacterial cytochrome ) NIR difference spectrum is similar to that of Fd.The NIR difference spectra of the electron-transfer cofactors were compared between different organisms and to those previously measured in vivo, whereas the relative absorption coefficients of all cofactors were determined by using single PSI turnover conditions. The fact that the NIR P700 coefficient is larger than that of PCy and much larger than that of other contributing species, combined with the observed variability in the NIR P700 spectral shape, emphasizes that deconvolution of NIR signals into different components requires a very precise determination of the P700 spectrum.Then: type the word you are searching for in the window that pops up!Additional research articles see Four distinct trimeric forms of light-harvesting complex II isolated from the green alga Chlamydomonas reinhardtii Light-harvesting complex II (LHCII) absorbs light energy and transfers it primarily to photosystem II in green algae and land plants.We used several preparation methods to obtain four distinct fractions (fractions 1–4), each of which contained an LHCII trimer with different contents of Type I, III, and IV proteins.The pigment compositions of the LHCIIs in the four fractions were similar.Snapshot transient absorption spectroscopy: toward in vivo investigations of nonphotochemical quenching mechanisms Although the importance of nonphotochemical quenching (NPQ) on photosynthetic biomass production and crop yields is well established, the in vivo operation of the individual mechanisms contributing to overall NPQ is still a matter of controversy.In order to investigate the timescale and activation dynamics of specific quenching mechanisms, we have developed a technique called snapshot transient absorption (TA) spectroscopy, which can monitor molecular species involved in the quenching response with a time resolution of 30 s.Noncyclic photophosphorylation is widely held to involve a collaboration of two photoreactions: a short-wavelength photoreaction belonging to System II and a long-wavelength photoreaction belonging to System I.Recent findings, however, indicate that noncyclic photophosphorylation may include two short-wavelength, System II, photoreactions that operate in series and are joined by a “dark” electron-transport chain to which is coupled a phosphorylation site.

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